Research Associate/Sr. Research Associate, Preclinical

Company: Synthego
Location: Redwood City
Posted on: June 6, 2021

Job Description:

Synthego is seeking an experienced, collaborative and highly motivated RA/SRA to join the Pre-clinical team. RA/SRA, Preclinical will be trained and provide support to senior scientists to advance a defined project from conception to proof-of-concept in vitro and in vivo. The successful candidate will have experience with molecular biology techniques such as NGS and ddPCR to evaluate on- and off-target analysis for genetically modified cell therapy products.

What You'll Do

• Prepare and QC NGS libraries.
• Operate and maintain MiSeq/NextSeq sequencing platforms.
• Operate and maintain ddPCR Instrument.
• Performs CRISPR/Cas9 gene editing on primary cells and/or established cell lines.
• Maintain and expand primary cells
• Flow cytometry analysis to phenotype engineered primary cells.
• Test conditions to optimize gene editing of specific cell type
• Assist in tissue processing and analyses

About You

• BS or MS in molecular biology, cell biology, gene therapy or related discipline, with a minimum of 3+ experience in academia or industry setting, with strong motivation.
• Experience preparing and QC (Fragment analyzer, Qubit, etc.), of medium to high volume NGS library preparation.
• Experience operating MiSeq or NextSeq platforms is required.
• Direct experience with molecular biology techniques, including but not limited to mRNA and DNA prep, PCR, RT-PCR, qPCR.
• Sterile cell culture technique is desired
• Experience running (required) and analyzing (desired) droplet-digital PCR.
• Detail-oriented, organized, ability to implement protocols and keep good notes in a lab notebook is a must.
• Previous experience with multicolor flow cytometry, cell based assays, ELISA is a plus
• Experience with primary cell isolation (HSC, T cell, B cell, monocytes, et al) and engineering using viral and/or nonviral approach is a plus
• Familiarity with NGS data analysis using R or Python a strong plus
• Ability to present and communicate project updates at team meetings
• Ability to work within a fast-paced environment, with "Can do" and "Do now" attitude
• Demonstrated ability to perform as a team player

Company Perks & Benefits

• Competitive salary
• Generous equity at a rapid growth company
• Medical, dental, and vision benefits
• 401k Plan
• Free catered lunches and dinners with fully stocked kitchen & snacks
• Onsite Gym
• Daily Shuttle bus to & from San Francisco
• Paid parental leave
• Flexible paid time off
• Learning and Development resources

About Us

Synthego is a genome engineering company that enables the acceleration of life science research and development in the pursuit of improved human health.

The company leverages machine learning, automation, and gene editing to build platforms for science at scale. With its foundations in engineering disciplines, the company's platforms vertically integrate proprietary hardware, software, bioinformatics, chemistries, and molecular biology to advance basic research, target validation, and clinical trials.

By providing both commercial and academic researchers and therapeutic developers with unprecedented access to cutting-edge genome engineering, Synthego is at the forefront of innovation, accelerating the development of truly engineered biology.

Keywords: Synthego, Redwood City , Research Associate/Sr. Research Associate, Preclinical, Other , Redwood City, California