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Senior Research Associate, Protein Purification

Company: Bevov
Location: Redwood City
Posted on: July 18, 2021

Job Description:

Job Description

Our Client is looking for an experienced protein biochemist to join the company’s drug discovery efforts. S/he is expected to play a central role in efforts to identify and progress lead LYTAC programs through their work in bioconjugation. A successful candidate has a solid base in amino acid chemistry and working with recombinant proteins and antibodies. This includes protein purification and the synthesis and analysis of antibody-drug conjugates (ADCs). A broad scientific background with a recent emphasis on ADCs may be ideal as occasional forays outside the central role of bioconjugation are likely.

Responsibilities

  • Conduct antibody conjugation reactions at small and large scale using commonly employed lysine and cysteine chemistry.
  • Optimize synthetic procedures and purification protocols for ADCs to maximize both the efficiency of conjugation and product stability.
  • Analytical characterization of ADCs and naked Mabs by LC-MS, SEC, IEC, and SDS-PAGE.
  • Assess binding characteristics ADCs in vitro.Occasional assessment of ADC bioactivity in cell-based assays.
  • Maintain an accurate database of ADCs.
  • Analyze, document, and report data.
  • Present unbiased results in regular group meetings

Qualifications

  • BS/MS in biochemistry or related field with a minimum of 2 years experience in the chemical conjugation of proteins and 7+ years total lab experience.
  • Experience with intact mass analysis of proteins and/or ADCs by LC-MS.Use of AKTA systems for protein purification via SEC, IEC, and affinity chromatography.
  • Experience with analytical SEC and reverse phase chromatography (HPLC).
  • Sound understanding of biological buffers and the effect of pH on amino acid chemical reactivity.
  • Sound understanding of protein stability and the ability to determine when a reagent (protein or ADC) is compromised.
  • Experience with endotoxin testing and prevention of endotoxin contamination of equipment and reagents.
  • Basic tissue culture skills and understanding of data generated in cell-based functional assays.
  • Experience with electronic notebooks.
  • Experience with Biacore or Octet desired but not required

Benefits

Our client is an equal opportunity employer and value diversity at their company. They do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status, veteran status, or disability status

Keywords: Bevov, Redwood City , Senior Research Associate, Protein Purification, Other , Redwood City, California

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